VWR will support you from the latest life science products to the guaranteed purity of organic building blocks. Mixing of incompatible materials (chemicals or wastes) can result in excessive heat, over pressurization, fire or other dangerous situations. The high flow agarose matrix provides particle rigidity without. This method enabled > 90 % overall recovery of unformulated DS at ≥ 150 g/L.A strong, vibrant research and development group is the lifeblood of all industries. The ligands, Q, S and DEAE, are coupled to a chemically modified, high flow agarose matrix. Most of the rinse was combined with the retentate to hit the target protein concentration. After the retentate was collected, a minimal volume of buffer was added for the UF rinse. During the UF/DF process, the antibody was initially concentrated to 90 g/L, diafiltered, and concentrated to ≥ 180 g/L, then the retentate was collected. Analysis showed that the Capto S run removed the excipients with yields of ≥ 96%. Capto S is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. CAPTO-COMPATIBLE COLLET CHUCKS C4 ER COLLET CHUCKS Part No. Efficient aggregate removal at high load of monoclonal antibodies. High binding capacity, > 100 mg MAb/mL medium. High binding capacity, > 100 mg mAb/mL resin. Capto S ImpAct is a strong cation exchange chromatography medium (resin) developed for intermediate purification and polishing of monoclonal antibodies (MAbs) and other biomolecules. If you plan to mix chemicals or wastes in a waste container or group them in a bag, tray or bucket, you must first determine whether any dangerous situations may result. Overview Specifications Related Documents Order Table Product Support With a high binding capacity and small particle size, Capto S ImpAct is a good choice for reliable and robust polishing steps in an industrial purification process. Analysis showed that the Capto S run removed the excipients with yields of 96. Mixing of incompatible materials (chemicals or wastes) can result in excessive heat, over pressurization, fire or other dangerous situations. In addition, Capto S has lower resin costs, takes less time to process, and uses milder elution conditions. In addition, Capto S has lower resin costs, takes less time to process, and uses milder elution conditions. Capto S withstands effective and rigorous CIP procedure and the chemical stability ensures long media lifetime. A Capto S column was chosen over Protein A chromatography to remove excipients from formulated drug substance because of its higher binding capacity. PreDictor Capto S filter plates 96-well plates for high-throughput screening or process development. Capto S is available in the following formats: Process development scale prepacked. Chemical stability Stable to commonly used aqueous buffers, 1.0 M NaOH5, 1. A Capto S column was chosen over Protein A chromatography to remove excipients from formulated drug substance because of its higher binding capacity. Capto S withstands effective and rigorous CIP procedure and the chemical stability supports long resin lifetime. Fortunately, a sufficient supply of formulated DS was available for reprocessing. Capto S is a strong cation exchanger for fast, efficient, and cost-effective capture and intermediate protein purification from large feed volumes. Since the pilot plants were not available for large-scale campaigns, a creative alternative was needed to produce 2 kg of antibody from formulated DS for these studies. During process scale-up, the project team decided to make a high-concentration mAb drug substance for subcutaneous injection and change the formulation. The full offering is listed in Product sheet 29161435. High quality columnexcellent reproducibility, durability, and high chemical and pH stabilities. The scope of this work is two-fold: 1) excipients removal from formulated mAb drug substance by Capto S chromatography and 2) UF/DF process development to make high-concentration drug substance (DS) for subcutaneous injection. BioProcess resin groups available in the ReadyToProcess format are MabSelect PrismA, MabSelect SuRe, MabSelect, Capto S ImpAct, Capto ImpRes, Capto, Sepharose 6 Fast Flow, and Sepharose High Performance resins. Capto HiRes S 10/100 find -GE29275879 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
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